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橙色熒光蛋白激發(fā)光譜

作者:上海峰志 時間:2021-04-04 11:27:43瀏覽2927 次

信息摘要:

mOrange熒光蛋白是2004年發(fā)表的一種堿性(組成型熒光)橙色熒光蛋白,來源于Discosoma sp.。它具有高酸敏感性。mOrange is a basic (constitutively fluorescent) orange fluorescent protein published in 2004, derived from Discosoma sp.. It has high acid sensitivity.

橙色熒光蛋白mOrange激發(fā)光譜

mOrange是2004年發(fā)表的一種堿性(組成型熒光)橙色熒光蛋白,來源于Discosoma sp.。它具有高酸敏感性。mOrange is a basic (constitutively fluorescent) orange fluorescent protein published in 2004, derived from Discosoma sp.. It has high acid sensitivity.

morange橙色熒光蛋白的激發(fā)光的中心波長為548nm,發(fā)射光的中心波長為562nm。

橙色熒光蛋白mOrange激發(fā)光譜圖:

morange.png

Orange fluorescent protein Proteins

Orange fluorescent protein (OFP) is a versatile biological marker used to monitor physiological processes, visualize protein localization, and detecting transgenic expression in vivo. OFPSparkTM is a red (orange) fluorescent protein (excitation/emission maxima are 549 and 566 nm, respectively) derived from DsRed. Possessing high photostability and pH stability, OFPSparkTM is more than twice brighter than mOrange2. OFPSparkTM can be expressed and detected in a wide range of organisms.

橙色熒光蛋白 (OFP) 是一種多功能生物標(biāo)記物,用于監(jiān)測生理過程、可視化蛋白質(zhì)定位和檢測體內(nèi)轉(zhuǎn)基因表達。OFPSparkTM 是一種源自 DsRed 的紅色(橙色)熒光蛋白(激發(fā)/發(fā)射zui大值分別為 549 和 566 nm)。OFPSparkTM 具有高光穩(wěn)定性和 pH 穩(wěn)定性,比 mOrange2 亮兩倍以上。OFPSparkTM 可以在多種生物體中表達和檢測。

什么是mOrange2熒光蛋白?

mOrange2 fluorescent protein. Living Colors mOrange2 is an extremely bright orange fluorescent protein monomer ideal for use as a tag or reporter. The mOrange2 fluorescent protein mutant derived from a monomeric mutant of DsRed, which was developed in Dr. Roger Tsien’s lab (Nathan C Shaner et al. 2004; Wang et al.

常用熒光蛋白的激發(fā)和發(fā)射光:

熒光蛋白蛋白型號激發(fā)波長(EX)發(fā)射波長(EM)

青色熒光蛋白
Cyan fluorescent proteins

AmCyan1458nm489nm
綠色熒光蛋白
Green fluorescent proteins
AcGFP1
475nm505 nm
ZsGreen1493nm505nm
黃色熒光蛋白
Yellow fluorescent proteins
ZsYellow1529nm539 nm
mBanana540nm553 nm
桔色熒光蛋白
Orange fluorescent proteins
mOrange2549nm565nm

紅色熒光蛋白
Red fluorescent proteins 

DsRed-Express2554nm591nm
DsRed-Express554nm586nm
tdTomato554nm581 nm
DsRed-Monomer557nm592 nm
DsRed2563nm582 nm
AsRed2576nm592 nm
mStrawberry574nm596 nm
mCherry587nm610nm


EX: Excitation max (nm)

EM: Emission max (nm)

如果您想觀察熒光蛋白在動植物上的表達,建議使用便攜式激發(fā)光源,上海峰志儀器有限公司銷售便攜式雙波長熒光蛋白激發(fā)光源,具體詳細介紹請瀏覽《便攜式熒光蛋白激發(fā)光源LUYOR-3415RG》。推薦使用LUYOR-3415便攜式雙波長激發(fā)光源或《LUYOR-3280熒光手電筒》,這樣您就可以用便攜式熒光蛋白激發(fā)光源直接觀察熒光蛋白在植物的種子、愈傷、根系和葉片上的表達。上海峰志儀器有限公司免費提供便攜式熒光蛋白激發(fā)光源樣機試用,滿意付款,具體請按照網(wǎng)頁底部聯(lián)系信息電話或.聯(lián)系。

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